Dr. Nicholas Boyd Gibbins

Dr. Nicholas Boyd Gibbins (MEng, PhD)

Scientific Director – SC21: 

Dr. Nicholas is the Scientific Director for SC21 Group in Bangkok, Thailand. He is an experienced bioengineer specializing in, human iPS cell culture, neural organoids, cancer organoid modeling, tumor microenvironment, and CRISPR-Cas9 genome editing.

His placements included Queen Mary University in London, University College of London, and CiRA, Kyoto University, Japan – trained by noble prize winner Shinya Yamanaka.

Dr. Nicholas Boyd Gibbins (MEng, PhD)

Research Experience

Postdoctoral Research Fellow
(2017 – 2019)
Department of Clinical Application, CiRA, Kyoto University, Japan

Summary:

• Developing a high throughput 3D organoid culture screening system for Diffuse Intrinsic Pontine Glioma (DIPG) by CRISPR-Cas9 gene editing of human iPS cells to create cancer cell line models.
• Designed and performed CRISPR-Cas9 RNP knock-out and ssODN knock-in genome editing experiments to develop cancer models by electroporation.
• Differentiated iPS cells into neural stem cells, OPCs, neurons, oligodendrocytes, and glial cells, with desired brain regional specificity
• Optimized 3D organoid culture for 96-well plate format
• Established overexpression cell lines using the piggyBac transposon system.
• Supervised and lead project of full-time technician to strategically improve organoid system to incorporate key human cancer interactions of the tumor microenvironment.

Ph.D Student
(2013 – 2016)
Albert Lab, Ear Institute, University College London

• PhD Project focused on homeostasis and protein turnover in the auditory transducer complex of Drosophila melanogaster, as well as its molecular, cellular and system-level characterization, and functional genomic analysis of the mode of action of neuroactive insecticides.
• Established conditional gene regulation systems
• Characterized proteostasis / homeostasis in Drosophila hearing
• Succeeded in achieving adult hearing regeneration in deaf mutant background.
• Electrophysiology
• Functional genomic screening in collaboration with Syngenta for insecticide mode of action analysis.

Master’s student
(2011 – 2012, 8 months)

Master’s Group Project: The Design of an Ankle Rehabilitation Device, Queen Mary London

Group project to design a rehabilitation device for chronic ankle instability sufferers, specifically aiming to re-train vestibular control after proprioceptive disruption.
• Took a leadership role to organize group activities

DR.

NICHOLAS

RESUME

SUMMARY

Education

Ph.D. in sensory neuroscience
University College London: 2013-2017

Master’s in Medical engineering (Meng 4 years)
Queen Mary University, London: 2008-2012
• First class with honours
• Academic award for third year dissertation project

Undergraduate Student
(2010 – 2011, 8 months)

Independent Project under the supervision of Prof David Lee, Queen Mary London

• Research project focused on reprogramming MEFs into iPSCs and silencing the expression of structural proteins from the nucleus in order to investigate the role of mechanical signals
in differentiation.
• iPS cell culture
• siRNA-mediated gene KO
• RT-qPCR, flow cytometry and FACS, ICC, confocal microscopy and other basic lab techniques

• Molecular biology
• Cell culture (e.g. iPSCs,
organoids)
• piggyBac Transposons
• siRNA-mediated knockdown
• CRISPR-Cas9 gene editing
• Functional genomic screening

• High throughput drug screening
• RT-qPCR
• Confocal microscopy
• Transfection (lipids and
electroporation)
• Cell sorting
• Sanger Sequencing

• Academic award for third year dissertation project (Queen Mary)
• UCL/Syngenta Impact PhD studentship award ($75,000 over 3 years)

• Faculty of Brain Sciences Postgraduate Poster & Presentation Symposium – first place (UCL)
• Bogue Fellowship collaborative research grant to work in the USA ($4000, UCL)
• UCL Graduate School SLMS External Training Fund ($1000, UCL)
• 31st Microelectrode Techniques for Cell Physiology Workshop bursary ($400,Marine Biological Association)

• Faculty of Brain Sciences Postgraduate Poster & Presentation Symposium – first place (UCL)

• English (native)
• Japanese (basic)

Matthew P Topping, Marta Andres Miguel, Nicholas Boyd-Gibbins, Jason Somers, Joerg Albert “Sex and Species Specific Hearing Mechanisms in Mosquito Flagellar Ears” Nature Communications 2018 September 25;9(1):3911

[In preparation] Boyd-Gibbins N*, Tardieu C*, Gale J & Albert JT Homeostasis and protein turnover in the auditory transducer complex of Drosophila melanogaster. [*denotes equal contributions]

[In preparation] Shao-Kuei Huang, Boyd-Gibbins N, Albert JT, Kernan MJ
“Regulated alternate splicing changes the TRPN channel pore to diversify
mechanoreceptor function in insects”