Dr. Rajneesh Verma

Dr. Rajneesh (Raj) Verma (BSc, MSc, PhD)

Head of iPSC department: 

Dr. Raj heads the iPSC department at the SC21 Group in Bangkok, Thailand. His vast experience in the iPSC field of science has allowed the iPSC21 program to be developed into a commercially available iPSC biobank.

In parallel to our commercial ready iPSC banking and iPSC-MSC differentiation service, Dr. Raj is actively researching new and exciting ways to utilize the immortal iPS cell lines produced.

Dr. Rajneesh Verma BSc, MSc, PhD – CV

Research Experience

2016 – ongoing Chief Scientific Director at SC21 Group / Stem Cells 21 Co. Ltd, Bangkok, Thailand.
Duties: Making and Biobanking of Clinical Grade iPSC and expand the differentiated cells in Bioreactor for clinical applications

2017- 2019 Program Specific Researcher Centre for iPS Research and Application(CIRA), Kyoto University, Kyoto, Japan.
Duties: Clinical Grade iPSC to erythrocytes for sickle cell anemia, malaria and Thalassemia patients and expand the cells in Bioreactor for clinical applications

2014- 2015 Foreign Expert, Program Leader in Livestock and Endangered species (iPSC &IVF), Mahidol University , Bangkok, Thailand and Adjunct Senior Lecturer in Monash Institute of Medical Research, Melbourne, Australia.
Duties: iPSC , Genome-Editing (TALENS/ CRISPR) and ART in endangered species and IVF in Endangered and livestock species.

2010-2014 PhD on Wild Cats iPS cells, MIMR, Monash University, Melbourne, Australia.


2009 Senior Research Assistant, MIMR Melbourne, Australia
Duties: Generating iPS cells from Mouse, Bovine, Horse, Human, Sheep and characterizing them. And teaching/training IVF to master’s in reproductive biology students of embryology department.

2008 Senior Research Assistant Monash Antibody Technology Facility (MATF), Melbourne, Australia.
Duties: Monoclonal Antibody production, ELISA, Operation of Robots.

2007 Experimental Scientist-Cell Biologist Centre for Molecular Health and Technologies CSIRO, Melbourne, Australia
Duties: Isolation of Spermatogonial stem cells/Sertoli from bovine testes, Immunohisto-chemistry, Western blot, cell culture, developing new methods of isolation of sertoli/myoid cells from testes, determining the ECM matrix proteins secreted from the cells derived from the testes. And expanding the stem cells using spinner culture, Wave bag, and bioreactor.

2006 Senior Research Associate and Laboratory Manager Centre for Reproduction and Development, Monash Institute of Medical Research (MIMR), Melbourne, Australia
Duties: Isolation of stem cells from Bovine and Murine, Training Master’s student for IVF in Bovine and Mouse, Managing stem cell and embryology Laboratory. RT-PCR on ES cells.

2005 Senior Research Associate Centre for Functional Genomics and Human Disease, Monash Institute of Medical Research (MIMR), Melbourne, Australia
Duties: PCR, Immunohisto-chemistry, Looking after breeding colonies of mice, DNA and RNA extraction, Growing Embryonic stem cell, Growing Tumor cells and injecting into the mice, Biochemical assays, FACS analysis, and Cryosectioning.

2004 Senior Research Associate Centre for Early Human Development, MIMR, Melbourne, Australia
Duties: PCR, Immunostaining, Operting Confocal microscope, Plasmid isolation, Transformation, and Transfection. RNA isolation, Isolation of Fibroblasts from mice, cloning, FACS analysis, Maintaining mice colonies, Immunostaining, and IVF

Visiting Scientist (Feb-April 2004) Melbourne University, Zoology Department, Australia
Duties: Analysing Microarray data using Bioinformatics, hybridization of microarray slides and RT-PCR.

2003 Research Assistant C.S.I.R.O, Canberra, ACT, Australia.
Duties: Transformation, Plasmid isolation an Analysing Microarray data using Bioinformatics and hybridization of microarray slides and RT-PCR in Rice.

2001 Technical Officer University of Sydney, Plant Breeding Institute, Cobbitty, Sydney, Australia
Duties: Spectrophotometric analysis on wheat flour, Management of Quarantine, Sowing, Harvesting, Threshing, Taking care of plants in microclimate, Chemical spraying, and Data entry

2000 Tutor Victoria University of Technology, Melbourne, Australia
Duties: Teaching 1st-year Biology, Chemistry, and Calculus

DR.

RAJ

RESUME

SUMMARY

  • 2014    PhD in using reproductive technologies and iPS Cells to save endangered wild cats, Monash University, Melbourne.
  • 2003 Masters in Plant Breeding/Agriculture “Molecular mapping of Low temperature male sterility in rice using Micro satellite markers.” University of Sydney, Plant Breeding Institute, Sydney.
  • 2000 Bachelor in Applied Biology (Biotechnology).
  • Victoria University of Technology, Melbourne
  • 2009  Advanced rodents handling and surgery, Monash University
  • 2006 Occupational Health, Safety, and Environment, Monash   University
  • 2002 Senior First Aid Officer, Red Cross Australia
  • 2001 Farm Chemical User Accreditation. Chem-Cert Australia
  • Best Speaker in 4th International Stem Cell Conference in New Delhi , India (2018)
  • Best Speaker in International Stem Cell Conference at Mahidol University , Bangkok, Thailand (2015)
  •  AMITY University Memento in Stem cell Symposium, New Delhi, India(2014)
  •  Nobel India magazine awarded the “Youngest achiever” of India by the Governor of Uttar Pradesh, India (2013)
  •  Lucknow mahotsav event by Govt. of India awarded the “High Achiever” of India by Chief Minister of Uttar Pradesh, India (2013)
  •  Awarded Youngest Indian Scientist in Australia, Melbourne, Victoria  (2012)
  •  Nominated for the Young Scientist of Australia, Melbourne, Victoria  (2012)
  •  First Prize in the PhD students Annual Symposium, MIMR, Melbourne (2012)
  •  First Scientist in the world to make stem cells from Snow Leopard (2012)
  •  Victorian PhD Scholarship (MIMR) Monash University, Melbourne (2009)
  •  South Australian PhD scholarship (faculty of health science) from University of Adelaide (2008).
  •  Awarded First prize on the poster competition at Dairy CRC Annual Conference in Queensland, Australia (2007).
  •  Completion of Master’s degree by High Distinction (Topper) in 2002. University of Sydney
  •  Rice CRC Honours scholarship. Rice CRC, Australia 2002.
  •  Supplementary scholarship for Master’s in Agriculture in 2002. University of Sydney
  •  Pest Animal Control Honours scholarship in 2002. CSIRO Animal Reproduction Unit, Canberra.
  •  IASTE Studentship for Technical Experience. Forest Genetic Department, University of Gottingen, Germany 2001
  •  Undergraduate Summer Vacation Scholarship. Quality Wheat CRC, Australia  2000
  •  Best Biotechnology student of the year 1998, 1999 & 2000. Victoria University of     Technology. Melbourne

Tissue culture techniques, Isolation of animal and plant genomic DNA, Microsatellite and Mapping, DNA& RNA purification, Southern Blotting, Northern Blotting, SDS-PAGE gel,Cloning in various vectors, Real-Time PCR, Animal Cell Culture, Plant Cell Culture, Spectrophotometric analysis, Animal dissection, ELISA assays, Microbiology Techniques, Confocal Microscope, Western blot, mammalian cell culture, IVF, ICSI, microarrays, iPS Cells generation, Reproductive and molecular cloning , genome-editing and Immuno-staining.

  • Verma, R , Zhao, X.., Dolferus, R., Darvey, N., and Dennis, E.S. (2002). “Chromosomal location of QTLs controlling low temperature induce male sterility in temperate Japonica rice” Plant breeding journal, Vol 62, University of Sydney
  • Lim ML, Vassiliev I, Richings NM, Verma R, Firsova A, Zhang C and Verma PJ. (2011) A novel, efficient method to derive bovine and mouse embryonic stem cells with in vivo differentiation potential by treatment with 5-azacytidine. Theriogenology; 76(1):133-42..
  • Verma R, Holland MK, Temple-Smith P, Verma PJ. (2012) Inducing pluripotency in somatic cells from the snow leopard (Panthera uncia), an endangered felid. Theriogenology 77(1):220-8,.
  • Verma R, Liu J, Holland MK, Temple-Smith P, Williamson M and Verma PJ. (2013) Nanog is an Essential Factor for Induction of Pluripotency in Somatic Cells from Endangered Felids. (2013) BioRes Open Access. 2(1):72-6. doi: 10.1089/biores.2012.0297
  • Verma R, Ghahfarokhi T Amir, Suprakob G Rachanont and Verma PJ. (2018) Oct-4, the Most Important Pluripotency Gene: Cloning, Characterization and Bioinformatics Analysis in Endangered Wild Cats; Bengal Tiger, Snow Leopard and Jaguar. Journal of Stem Cell and Transplantation Biology, ISSN: 2469-5157. Open Access, Elyns group
  • Verma R, Shelar Yogesh, Venkatesh M.P and Bansode M(2019) Overview of current regulatory practices, challenges & guidance for regulation of ”(stem cells and stem cell derived products) in india. Cient Periodique Journal, Volume 6 , Issue 6, Open Access
  • Verma R, Shelar Yogesh, Venkatesh M.P and Bansode M(2019) “WHO Pre-Qualification for Vaccine Manufacturing”. Cient Periodique Journal, Volume 6 , Issue 6, Open Access
  • Verma R, Shashi Paul, Balamuralidhara V and Bansode M(2019) “Regulatory Requirements and Approval Procedure of Combination Profucts (Medical Devices) in India”. Cient Periodique Journal, Volume 7 , Issue 2, Open Access

Springer Book Chapter

  • Verma R and Verma PJ. (2014) Stem cells in animal species from pre-clinic to biodiversity. Springer. Humana Press NY. (published June 2014)
  • Heffernan C, Sumer H, Verma R , Liu J, Malver L, Carlvaho E, and Verma PJ.   (2013) Transcriptional  and Translational Regulation of Stem Cells. Advances in Experimental Medicine and Biology. Volume 786. Springer, Humana Press NY. USA
  • Heffernan C, Sumer H, Verma R and Verma PJ. (2009) Generation of clinically relevant pluripotent cells. In Pluripotent Stem Cells. Springer,  Ed: Columbus F. Nova Publishers, NY. USA.